The Department of Chemistry and Biochemistry occupies space in the Barnes Science Center, built in 1988, and in Olin Hall, built in 1961 and later remodeled in 1988. The department has at its disposal roughly 20,200 square feet of physical space between the two buildings with an additional 1,000 square foot instrument laboratory housed in the recently constructed (2003) Tutt Science Center.

The department is home to an elaborate collection of state-of-the-art instrumentation for undergraduate use. Recent acquisitions include a Bruker AVANCE NEO 400 MHz NMR with autosampler (2022), Renishaw inVia confocal Raman microscope (2021), TA Discovery 250 Differential Sanning Calorimeter with autosampler (2021), TA Discovery 5500 Thermogravemetric Analyzer with autosampler (2021), and JASCO J-1100 Circular Dichroism Spectropolarimeter (2021). See below for a complete list of instrumentation.

There is dedicated faculty research space located in both the Barnes Science Center and Olin Hall. Most research labs are equipped with fume hoods, standard laboratory equipment, chemical and solvent storage, and desk space for student use. Furthermore, machine, glass-blowing, and electronics shops are accessible through the Departments of Chemistry and Physics.